In realtime pcr, cyclebycycle detection of accumulated pcr. Refurbished applied biosystems abi 7900ht fast sequence detection system real time pcr system pn 4330966 complete with sample block, pc, and abi 7900ht sequence detection system software. Used applied biosystems abi prism pcr 7000 sequence. Available realtime rtpcr cycler and kinetic pcr machines. Contacts applied biosystems anthony petrucci, 5083837961 media anthony. Applied biosystems 7900ht sequence detection system. Applied biosystems quantstudio 1 realtime pcr systems.
Using sequence specific primers, the number of copies of a particular dna or rna sequence can be determined. System autoscanxt lcm image analysis software copycaller software. Applied biosystems analysis modules for qpcr and sanger. Applied biosystems 7500 realtime pcr system,the applied biosystems 7500 realtime pcr system is a versatile, leading edge platform offering enhanced performance capabilities and a future upgrade to. The applied biosystems steponeplus realtime pcr system, makes it simple and easy to get highquality realtime pcr results.
Applied biosystems abi stepone well realtime pcr system with. The applied biosystems 7900ht fast realtime pcr system is a high. A realfast and real versatile approach to realtime pcr. Applied biosystems dna analyzer we set a new standard in capillary electrophoresis with the 24capillary 3500xl genetic analyzer.
Relative quantitation rq algorithms in applied biosystems realtime pcr systems software. The joint solution will be comarketed exclusively by the two companies. Applied biosystems 3500 series genetic analyzers for dna sequencing and. Applied biosystems quantstudio 6 and 7 pro realtime pcr systems applied biosystems quantstudio 6. Applied biosystems stepone and steponeplus realtime pcr systems remarkably simple systems.
Applied biosystems 7500 fast, 7500 and 7300 realtime pcr. This instrument is capable of quantitative detection using realtime analysis. In realtime pcr, cyclebycycle detection of accumulated pcr product is made possible by combining thermal cycling, fluorescence detection, and applicationspecific software in a single instrument. Utilizing robust led based 4color optical recording, the steponeplus realtime pcr system is designed to deliver precise, quantitative realtime pcr results. Jul 25, 2018 applied biosystems microsatellite analysis software thermo fisher scientific. We found that gqpcr had the highest concordance with sequence analysis and the highest detection rate for mixed genotype detecting. Applied biosystems 7900ht sequence detection system grizzly. Applied biosystems stepone steponeplus realtime pcr systems. Built on more than 20 years of experience, applied biosystems taqman assays are designed to be sensitive, specific, and easy to use. Applied biosystems 7500 realtime pcr system thermo fisher.
Certain applied biosystems pcr reagents are developed and manufactured by roche molecular systems, inc. Applied biosystems high resolution melt curve analysis module 9. Applied biosystems steponeplus realtime pcr system. The upgrade to the enterprise edition software includes access to two new analysis packages designed for highthroughput snp genotyping snp manager and gene expression. Comparison of a novel realtime pcr assay with sequence. Plexor hy system for the applied biosystems 7500 and 7500. Prior to downloading any software to a computer used for instrument controloperation, please consult. Plate setup wizards for easy experimental design, even with complex. We compared a novel realtime genotyping and quantitative pcr gqpcr assay, direct sequence analysis, reverse hybridization, and multiplex pcr for genotyping hepatitis b virus hbv in 127 hbv. This is a secondgeneration sequence detection system designed for automated, highthroughput detection of fluorescent pcrrelated chemistries. Applied biosystems 7500 fast realtime pcr system thermo.
Prior to downloading any software to a computer used for instrument controloperation, please. The applied biosystems 7500 and 7500 fast realtime pcr systems are integrated, versatile platforms for the detection and quantification of nucleic acid sequences. Applied biosystems dna sequencing analysis software verion 5. Applied biosystems high resolution melt curve analysis. Prior to downloading any software to a computer used for instrument controloperation, please consult with your service and support. Check out the most popular applied biosystems products for genetic analysis. Audience this guide is intended for novice and experienced users who analyze. The abi prism 7000 sequence detection system qpcr rtpcr 7000 sds instrument is a secondgeneration sequence detection instrument capable of quantitative and qualitative detection with fluorescentbased pcr chemistries.
Find software downloads, patches, and updates for the instruments we offer. The applied biosystems developed taqman gene expression assays, a genomewide collection of quantitative, standardized assays for gene expression analysis by realtime quantitative rtpcr. Real time pcr combines thermal cycling, fluorescence detection, and applicationspecific software to measure the cyclebycycle accumulation of pcr products in a singletube. Applied biosystems 7500 getting started pdf download. Refurbished applied biosystems abi 7900ht fast sequence detection system real time pcr system pn 4330966 complete with sample block, pc, and abi 7900ht sequence detection system. The applied biosystems 7300 real time pcr system uses fourcolor detection providing the flexibility to perform a variety of applications including gene expression analysis, pathogen quantitation, snp genotyping, and isothermal. Sequencing analysis software uses a basecaller algorithm that performs base calling for pure and mixed base calls.
The 7900ht contains the hardware used for thermal cycling and detection of fluorescent chemistries. Applied biosystems stepone realtime pcr system fisher. Taqman national center for biotechnology information. This unique solution integrates integromics realtime statminer software with any combination of applied biosystems line of realtime pcr systems. Relative quantitation rq algorithms in applied biosystems real time pcr systems software. The software analyzes, displays, edits, saves, and prints sample files that are generated from applied biosystems dna analyzers and genetic analyzers. Autoscanxt lcm image analysis software copycaller software. We compared a novel real time genotyping and quantitative pcr gqpcr assay, direct sequence analysis, reverse hybridization, and multiplex pcr for genotyping hepatitis b virus hbv in 127 hbvinfected patients. Apr 22, 2015 applied biosystems analysis modules bring together multiple data sets in one convenient place. Assisted tm calling ability to create and save analysis templates on.
The software provides projectbased analysis of realtime and endpoint data for various quantitative and qualitative pcr applications. The stepone realtime pcr system is a lowthroughput realtime pcr instrument perfect for both firsttime and experienced users. Applied biosystems stepone realtime pcr system getting started guide for standard curve experiments 4376784 4377736 applied biosystems stepone realtime pcr system installation, maintenance, and networking guide 4376782 4377798 applied biosystems stepone realtime pcr system installation quick reference card 4376783 4377792. The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well format. Applied biosystems 7500 fast realtime pcr system, laptop. This remarkably simple 96 well, 4 color, realtime pcr system is. Analysis feature of variant reporter, seqscape or genemapper software. Advanced software solutions reduce analysis time through a 2040% reduction in data footprint and realtime data export.
Utilizing robust led based 3color optical recording, the stepone realtime pcr system is designed to deliver precise, quantitative realtime pcr results for a. System requirements the following table summarizes the system requirements for. Applied biosystems steponeplus realtime pcr system, laptop. How do i add sample files to the abi prismsequencing analysis v.
This unit is in good working condition and will be tested before it ships. Neue applied biosystems realtime pcr analysis module. The stepone realtime pcr system can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. The applied biosystems 7300 realtime pcr system uses fourcolor detection providing the flexibility to perform a variety of applications including gene expression analysis, pathogen quantitation, snp.
The taqman assay utilizes the 5 nuclease activity of taq dna polymerase to cleave a fluorescently labeled probe fam tm labeled mgb. Sequence scanner software allows you to view, edit, print, and export data generated using applied biosystems genetic analyzer instruments after data has been. Instrument software for the applied biosystems 7300 realtime pcr system runs on the windows xp operating system and provides instrument control, data collection, and data analysis. Applied biosystems 7500 realtime pcr software downloads.
Applied biosystems and integromics to offer integrated. Help from within applied biosystems 75007500 fast real time pcr software v2. The applied biosystems 7300 real time pcr runs on its own software platform and quantitative results are available immediately, once pcr run is complete. The stepone realtime pcr system is a lowthroughput realtime pcr instrument perfect for both firsttime and. The applied biosystems 7500 and 7500 fast real time pcr systems are integrated, versatile platforms for the detection and quantification of nucleic acid sequences. Applied biosystems 7500 realtime pcr system thermo. This is a secondgeneration sequence detection system designed for automated, highthroughput.
This instrument is designed to support the demanding performance needs of validated and process controlled environments, while retaining the unsurpassed application versatility that life science researchers expect. The 7000 combines thermal cycling, fluorescence detection, and applicationspecific software in a single instrument. Applied biosystems stepone steponeplus realtime pcr. The abi prism 7000 sequence detection system is a complete, realtime pcr system that detects and quantitates nucleic acid sequences. The applied biosystems high resolution melting hrm software is the easiest to use melting analysis software enabling realtime pcr melt curve assays to be used more accurately for mutation scanning. Prior to downloading any software to a computer used for instrument controloperation, please consult with your service and support representative. Help from within applied biosystems 75007500 fast realtime pcr software v2. Use the table of contents in the left navigation pane to find your topic of interest, or enter. View online or download applied biosystems 7900ht user manual.
The accuseq realtime pcr detection software is part of a system that performs. Applied biosystems 7500 fast and 7500 realtime pcr. Notes applied biosystems 75007500 fast real time pcr system getting started guide for standard curve experiments. The great thing about the abi 7300 real time pcr is theres no need to purify pcr, run any gels, or implement post pcr alterations. Applied biosystems microsatellite analysis software thermo fisher scientific. Applied biosystems quantstudio 5 realtime pcr system applied biosystems quantstudio 6 flex realtime pcr system v1. The steponeplus realtime pcr system can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software.
Applied biosystems qpcr analysis modules within the thermo fisher cloud are web browserbased software tools that are flexible, fast and easy to use. Applied biosystems high resolution melt curve analysis module. Applied biosystems abi 7900ht fast sequence detection. Applied biosystems abi stepone well realtime pcr system. Applied biosystems creates new software development. Applied biosystems stepone realtime pcr system getting started guide for standard curve experiments 4376784 4377736 applied biosystems stepone realtime pcr system installation. This remarkably simple 96 well, 4 color, realtime pcr system is designed with a userfriendly, yet powerful, interface for researchers of all experience levels.
Applied biosystems 7300 realtime pcr system applied. Used applied biosystems abi prism pcr 7000 sequence detection. The quantstudio 5 realtime pcr system is compact in form, factory calibrated and is delivered in userfriendly packaging to enable fast and simple installation for immediate use. Realtime pcr is a variation of the standard pcr technique that is commonly used to. Applied biosystems offers a family of realtime pcr instruments with the 96well block format. Perform standard pcr in less than 2 hours and fast pcr reactions in less than 40 minutes intuitive, flexible software and wizards guide new users through their realtime pcr experiments.
The applied biosystems 7500 fast realtime pcr system represents the latest innovation in realtime. Powerful and userfriendly, sequence detection software includes the following features. Obtain longer read lengths, more highquality bases, and increased accuracy at the 5 end get increased accuracy in regions w. The applied biosystems 75007500 fast realtime pcr systems use the following dye sets for calibration. Sanger sequencing and fragment analysis software thermo. Applied biosystems 7900ht sequence detection system the 7900ht contains the hardware used for thermal cycling and detection of fluorescent chemistries. User bulletin applied biosystems realtime pcr systems july 2007 subject. Real time pcr is a variation of the standard pcr technique that is commonly used to quantify dna or rna in a sample. This online solution makes it easier to view, store, and analyze qpcr and sanger sequencing data. The applied biosystems analysis software is a secure web application for analysis of data that are generated on thermo fisher scientific realtime pcr instruments. Applied biosystems abi 7000 real time pcr 7000 sequence detection system with sds 1. This software enables you to basecall, trim, display, edit, and print data from our entire line of capillary dna sequencing instruments for data analysis and quality control. Intended for laboratory staff and principal investigators who perform experiments using the stepone or steponeplus system. Applied biosystems dna sequencing analysis software v5.
The upgrade to the enterprise edition software includes access to two new analysis packages designed for highthroughput snp genotyping snp manager and gene expression rq manager as well as secure, robust and scalable data management in an oracle database. The applied biosystems high resolution melting hrm software is the easiest to use melting analysis software enabling realtime pcr melt curve assays to be used more accurately for mutation scanning and genotyping. Researchers use applied biosystems integrated systems for sequencing, flow cytometry, and real. Agribusiness realtime qpcr ce sanger sequencing ce fragment. Cy3 dye, cy5 dye, fam dye, joe dye, ned dye, rox dye, sybr green dye, tamra dye, texas red dye, and vic dye. Applied biosystems 7300 real time pcr system the abi 7300 is a reliable tool which combines thermal cycling with fluorescence detection that measures cycle by cycle accrual. The pinnacle of automated purification for dna, rna, proteins, and cells. Applied biosystems quantstudio 5 realtime pcr system, 96. Applied biosystems stepone realtime pcr software stepone software.
Intended for laboratory staff and principal investigators who perform experiments using. Specifically designed with a user friendly, yet powerful interface, they are ideal for both new and experienced realtime pcr researchers. Applied biosystems dna analyzers are used for throughput genetic analysis, microsatellites, aflp, snp analysis, mutation detection and traditional dna sequencing find online auctions and classified ads. Applied biosystems 7300 real time pcr system andbio. Abi prism 7000 sequence detection system abi prism 7700. Abi prism 7000 sequence detection system abi prism. Applied biosystems instruments, assays, and reagents have been trusted in the lab for over 20 years. Applied biosystems analysis modules bring together multiple data sets in one convenient place.
Applied biosystems microsatellite analysis software youtube. Researchers use applied biosystems integrated systems for sequencing, flow cytometry, and realtime, digital and end point pcrfrom sample prep to data analysis. For host cell residual dna quantitation, a custom module built into the main. Realtime throughput computer desktop laptop or desktop applied biosystems 7500 fast realtime pcr systemstandard with rq standard with rqpaid options. Dec 15, 2007 this unique solution integrates integromics real time statminer software with any combination of applied biosystems line of real time pcr systems. Real time pcr is a laboratory method used to detect and determine the amount of nucleic acids present in samples. This applied biosystem prism 7000 sequence detection system is in excellent condition. Laboratory equipment measuring instruments control unit bicycle.